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Separation of amino acid phenylthiohydantoins by high-performance liquid chromatography on phenylalkyl support

โœ Scribed by Louis E. Henderson; Terry D. Copeland; Stephen Oroszlan

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
527 KB
Volume
102
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A high-performance liquid chromatographic analytical system is described for the separation, identification, and quantitation of phenylthiohydantoin (PTH) derivatives of all amino acids normally encountered in proteins. The system employs reverse-phase chromatography on a PBondapak phenylalkyl stationary phase. PTH derivatives of all amino acids can be identified and quantitated in a single 30-min analysis using linear gradient elution. The method can be applied equally well to the separation of methyl esters of the PTH-derivatized decarboxylic amino acids. The sensitivity of the method extends into the range of less than IO pmol.

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