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Complete phenylthiohydantoin amino acid analysis by high-performance liquid chromatography on ULTRASPHERE-octadecyltrimethyloxysllane

โœ Scribed by Ralph Somack

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
313 KB
Volume
104
Category
Article
ISSN
0003-2697

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All the organic soluble amino acid phenylthiohydantoins are eluted (but not totally resolved) in under 40 min on either a Zorbax ODS or Permaphase ETH column. The superior resolution of the Zorbax ODS column and the complimentary characteristic of the Permaphase ETH column allow the isocratic analys

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The phenylthiohydantoins (Pth) of the common amino acids can be resolved in a single analysis using a 25 x 0.46cm DuPont Zorbax cyanopropylsilane (CN) column developed with a gradient of methanol/acetonitrile (17:3) in sodium acetate buffer, pH 5.4. The Zorbax CN columns exhibit greater durability,

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