𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analysis of phenylthiohydantoin amino acids by high-performance liquid chromatography on DuPont Zorbax cyanopropylsilane columns

✍ Scribed by N.D. Johnson; M.W. Hunkapiller; L.E. Hood

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
284 KB
Volume
100
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

The phenylthiohydantoins (Pth) of the common amino acids can be resolved in a single analysis using a 25 x 0.46cm DuPont Zorbax cyanopropylsilane (CN) column developed with a gradient of methanol/acetonitrile (17:3) in sodium acetate buffer, pH 5.4. The Zorbax CN columns exhibit greater durability, reproducibility, and sensitivity than do columns with an octadecylsilane (C,,) support when used for Pth amino acid analysis in automated polypeptide sequencing.

πŸ“œ SIMILAR VOLUMES

Advances in the analysis of amino acid p
Advances in the analysis of amino acid phenylthiohydantoins by high performance liquid chromatography
✍ Carl L. Zimmerman; Ettore Appella; John J. Pisano πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 437 KB

All the organic soluble amino acid phenylthiohydantoins are eluted (but not totally resolved) in under 40 min on either a Zorbax ODS or Permaphase ETH column. The superior resolution of the Zorbax ODS column and the complimentary characteristic of the Permaphase ETH column allow the isocratic analys

Rapid separation of amino acid phenylthi
Rapid separation of amino acid phenylthiohydantoins by isocratic high-performance liquid chromatography
✍ G.E. Tarr πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 485 KB

All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects

A rapid method for the identification of
A rapid method for the identification of amino acid phenylthiohydantoins by high performance liquid chromatography
✍ FΓΆldi, J. ;Valentin, C. ;Cohen-Solal, M. πŸ“‚ Article πŸ“… 1980 πŸ› John Wiley and Sons 🌐 English βš– 141 KB πŸ‘ 2 views

Thin-layer chromatogram (6 x 6 cm) of the model mixture: (1) inorganic phosphate, (2) choline, (3) choline phosphate, (4) glycerophosphorylcholine, (5) glycerophosphate, (6) betaine. Solvent systems: First direction (vertical), methanol/water/conc. NH4 OH/chlorotorm/acetone (5 : 2 : 1 : 2 : 2); Seco