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High-performance liquid chromatography-based assay for the quantitation of protein maleylation at the picomole level

โœ Scribed by Martin N. McCall; Simon B. Easterbrook-Smith

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
404 KB
Volume
166
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A new HPLC-based assay for the direct determination of the extent of maleylation of proteins is described. The rationale is founded on the HPLC detection of maleic acid that results from cleavage of maleyl lysyls by low pH treatment. The method, employing a C-18 reverse-phase column eluted isocratically with 0.01 M acetic acid, pH 5.4, and absorbance monitored at 210 nm, is sensitive (lower limit 1 pmol) and linear in the range 1 pmol-0.5 mumol and allows recovery of the native protein.

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