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A high-performance liquid chromatography method for the assay of cytidine monophosphate-sialic acid synthetase

✍ Scribed by Charles R. Petrie III; Walter Korytnyk

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
350 KB
Volume
131
Category
Article
ISSN
0003-2697

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✦ Synopsis

An HPLC method has been developed for the assay of cytidine monophosphate-sialic acid synthetase (EC 2.7.7.43) using ion-pair chromatography and gradient elution. This procedure permits the assay of alternative substrates and inhibitors of the enzyme and is not subject to the limitation of the colorimetric method. The newly synthesized N-acetyl-9-deoxy-9-fluoro-D-neuraminic acid was found to be a good substrate of the enzyme with a Km of 6.35 mM as compared to 1.84 mM for N-acetylneuraminic acid.

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