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A General High-Performance Liquid Chromatography-Based Assay for the Hydrolysis of N-Acyl Glutamates

✍ Scribed by Rebecca S. Myers; Patrick T. Paton; Azar Dastgah; Jose R. Martell; Clifford E. Berkman

Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 66 KB
Volume: 275
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1999.4327

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✦ Synopsis

A rapid and simple HPLC assay has been developed to separate and quantify N-acyl glutamates and the corresponding carboxylic acids of the acyl moiety. This method was specifically developed to assay hydrolytic activity for glutamate carboxypeptidases. Although established assays for specific substrates of such enzymes exist, they may not be amenable for examining the hydrolytic activity of new substrate probes. This assay was developed to accommodate such probes.

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