A General High-Performance Liquid Chroma
A General High-Performance Liquid Chromatography-Based Assay for the Hydrolysis of N-Acyl Glutamates
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Rebecca S. Myers; Patrick T. Paton; Azar Dastgah; Jose R. Martell; Clifford E. B
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Article
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1999
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Elsevier Science
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English
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A rapid and simple HPLC assay has been developed to separate and quantify N-acyl glutamates and the corresponding carboxylic acids of the acyl moiety. This method was specifically developed to assay hydrolytic activity for glutamate carboxypeptidases. Although established assays for specific substra