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Cloning and expression in Saccharomyces cerevisiae of a β-amylase gene from the oomycete Saprolegnia ferax

✍ Scribed by Hee-Ok Kim; Jeong-Nam Park; Hyun-Jung Sohn; Dong-Jun Shin; Chan Choi; Suhn-Young Im; Hwanghee Blaise Lee; Soon-Bai Chun; Suk Bai

Book ID
110226782
Publisher
Springer Netherlands
Year
2000
Tongue
English
Weight
166 KB
Volume
22
Category
Article
ISSN
0141-5492

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✍ Kohchi, Chie ;Toh-e, Akio 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 553 KB

Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as a