Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as a
Cloning and expression of β-galactosidase gene from Streptococcus thermophilus in Saccharomyces cerevisiae
✍ Scribed by Byong H. Lee; Normand Robert; Chantal Jacques; Line Ricard
- Publisher
- Springer Netherlands
- Year
- 1990
- Tongue
- English
- Weight
- 494 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0141-5492
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Cellobiose, the last product in cellulose degradation, is converted into two molecules of glucose by a beta-glucosidase. S. cerevisiae does posses the structural gene for a beta-glucosidase, but it is very poorly expressed; we thus decided to isolate and characterize that of Kluyveromyces fragilis.
Regulated promoters have advantages over nonregulated promoters in that gene expression levels can be controlled through manipulation of environmental conditions. The ## Methods Yeast Strain and Plasmid use of regulated promoters in the design of plasmid molecules allows separation of a cell grow