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A gene encoding Achlya bisexualis β-amylase and its expression in Saccharomyces cerevisiae

✍ Scribed by Hee-Ok Kim; Jeong-Nam Park; Dong-Jun Shin; Hwanghee Blaise Lee; Soon-Bai Chun; Suk Bai

Book ID
118661017
Publisher
Springer Netherlands
Year
2001
Tongue
English
Weight
211 KB
Volume
23
Category
Article
ISSN
0141-5492

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Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as a