Cloning of two β-xylanase-encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae
✍ Scribed by M. Luttig; I.S. Pretorius; W.H. van Zyl
- Book ID
- 110369035
- Publisher
- Springer Netherlands
- Year
- 1997
- Tongue
- English
- Weight
- 355 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as a
Cellobiose, the last product in cellulose degradation, is converted into two molecules of glucose by a beta-glucosidase. S. cerevisiae does posses the structural gene for a beta-glucosidase, but it is very poorly expressed; we thus decided to isolate and characterize that of Kluyveromyces fragilis.