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Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae

โœ Scribed by Naoto Hirose; Masao Kishida; Haruhiko Kawasaki; Takuo Sakai


Book ID
114294485
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
405 KB
Volume
86
Category
Article
ISSN
0922-338X

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Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as a