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Cloning Mammalian Genes Using cDNA Expression Libraries in Saccharomyces cerevisiae

โœ Scribed by Stephen J. Elledge; Chang Bai; Michael C. Edwards


Book ID
115638526
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
614 KB
Volume
5
Category
Article
ISSN
1046-2023

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Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as a