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Cloning of porcine neuron growth inhibitory factor (metallothionein III) cDNA and expression of the gene in Saccharomyces cerevisiae

โœ Scribed by Sue-Hong Wang; Chung-Yu Chang; Chien-Fong Chen; Ming F Tam; Yang-Hsin Shih; Lih-Yuan Lin


Book ID
117522753
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
174 KB
Volume
203
Category
Article
ISSN
0378-1119

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Expression kinetics of the human Epidermal Growth Factor (hEGF) from the a-factor prepro region in a 2-pm based plasmid was studied in Saccharomyces cerevisiae. Production o f hEGF was highly medium dependent as a chemically defined, nonenriched media had a significantly lower yield than did enriche