Characterization of Hamster-Bovine Somatic Cell Hybrids by in Situ Hybridization and Chromosome Banding
β Scribed by Elena Raimondi; Alessandra Mezzelani; Bianca Castiglioni; Daniela Moralli; Giuseppe Rognoni; Luigi De Carli
- Book ID
- 114809670
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 311 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0018-0661
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In situ hybridization of hamsterlhuman hybrids with biotinylated human genomic D N A has revealed that human chromosomal DNA can integrate into the hamster genome and is not always cytologically detectable. This finding helps t o explain why discordancy can arise in gene mapping by failing to recogn
## Abstract Hybrid clones were obtained between a mouse cell line (3TP) and a temperatureβsensitive Chinese hamster cell line (K12) unable to grow at 40Β° because of a __ts__ defect apparently located at the G1/S transition. The great majority of hybrid clones grew at 40Β°, showing the __ts__ defect
Mouse/hamster somatic cell hybrids form a valuable resource for mouse gene mapping. Characterization of these hybrids by isozyme analysis can be technically demanding and time-consuming. Speciesspecific polymerase chain reaction (PCR), where a mouse gene but not its homolog in the hamster is amplifi