Characterization of mouse-hamster somatic cell hybrids by PCR: A panel of mouse-specific primers for each chromosome

✍ Scribed by Cathy Abbott

• Book ID: 104736589
• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 440 KB
• Volume: 2
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00353858

✦ Synopsis

Mouse/hamster somatic cell hybrids form a valuable resource for mouse gene mapping. Characterization of these hybrids by isozyme analysis can be technically demanding and time-consuming. Speciesspecific polymerase chain reaction (PCR), where a mouse gene but not its homolog in the hamster is amplified, can provide an alternative means of characterization. Mouse-specific primers have been designed for at least one gene on each of the mouse autosomes and the X Chromosome (Chr). Primers are chosen to correspond to untranslated regions of the mouse gene concerned, in order to decrease the chance of crosshybridization with the homologous hamster gene. These primer sequences are presented, together with the conditions for their use.