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Use of fluorescent in situ hybridization to detect chromosomal rearrangements in somatic cell hybrids

✍ Scribed by Dr. Amato J. Giaccia; James W. Evans; J. Martin Brown

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 364 KB
Volume: 2
Category: Article
ISSN: 1045-2257
DOI: 10.1002/gcc.2870020314

No coin nor oath required. For personal study only.

✦ Synopsis

In situ hybridization of hamsterlhuman hybrids with biotinylated human genomic D N A has revealed that human chromosomal DNA can integrate into the hamster genome and is not always cytologically detectable. This finding helps t o explain why discordancy can arise in gene mapping by failing to recognize small pieces of foreign DNA in the rodent genome. Fluorescent in situ hybridization allows one t o locate these fragments in rodent chromosomes visually and possibly to identify their chromosome of origin.

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