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Clarification of subtle reciprocal rearrangements using fluorescence in situ hybridization

✍ Scribed by Sullivan, Beth A. ;Leana-Cox, Julie ;Schwartz, Stuart

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
902 KB
Volume
47
Category
Article
ISSN
0148-7299

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✦ Synopsis

Fluorescence in situ hybridization (FISH) using chromosome-specific DNA libraries as painting probes was applied in the analysis of six subtle, balanced chromosome rearrangements. Both fresh and older slides, some of which had been previously G-banded, were used to determine if FISH could identify unambiguously very small amounts of translocated material. Our results indicate that this procedure can clearly and precisely distinguish the specific components of extremely subtle translocations, in different cell types, such as leukocytes, aminocytes, and chorionic villus, and irregardess of preparation age. This ability makes FISH a valuable tool in clinical cytogenetics for the confirmation of preliminary G-banded k w o t m s .

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