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An improved enzymatic radioassay for ribose 1-phosphate

✍ Scribed by R.Scott McIvor; Robert M. Wohlhueter; Peter G.W. Plagemann

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 436 KB
Volume: 127
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90157-9

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✦ Synopsis

A method for measuring ribose l-phosphate in cell extracts is described. Cell extracts are first fractionated on polyethyleneimine-impregnated cellulose columns to remove nucleoside and base components which otherwise interfere with the enzymatic assay. Ribose l-phosphate in the eluate is made limiting for the conversion of ['%]hypoxanthine to [i4C]inosine in the presence of purine nucleoside phosphorylase. Labeled substrate and product are then easily separated on boronate gel columns or by paper chromatography.

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