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An improved method for the preparation of [14C]pyridoxal 5′-phosphate

✍ Scribed by Alec Lui; Ronald Minter; Lawrence Lumeng; Ting-Kai Li

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
432 KB
Volume
112
Category
Article
ISSN
0003-2697

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✦ Synopsis

Pyridoxal Y-phosphate was synthesized by first oxidizing [Wlpyridoxine to [Wlpyridoxal with MnOp ["C]Pyridoxal, isolated by cation-exchange chromatography, was then reacted with ATP in the presence of rat liver pyridoxal kinase to form ["C]pyridoxal Y-phosphate. The rat liver enzyme was used instead of the Escherichia coli pyridoxal kinase because the latter is strongly inhibited by pyridoxal. [W]Pyridoxal Y-phosphate was purified by Sephadex G-10 chromatography. The yield of the present method is 75%. The radiochemical purity of [Wlpyridoxal 5'-phosphate produced is 2 95%.

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