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An enzymatic method for the preparation in high yield of [5-14C]- and [4,5,6-14C] glucose

✍ Scribed by J. P. Longenecker; J. F. Williams

Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
304 KB
Volume
18
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

[5‐^14^C]‐ and [4,5,6‐^14^C] glucose were prepared from [2‐^14^C]‐ and [U‐^14^C] glycerol and fructose 6‐phosphate by reactions involving the enzymes glycerokinase, glycerol 3‐phosphate dehydrogenase, triose phosphate isomerase, transaldolase, lactate dehydrogenase and phosphoglucose isomerase. The authenticity of each of the ^14^C‐labelled sugars was verified by chromatographic and enzymic procedures and the distribution of ^14^C isotope confirmed by specific degradative procedures. These specifically labelled substrates are required for the quantitative estimation of the new pentose pathway reactions in liver, photosynthetic and some tumour tissues.

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