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An enzymatic method for the synthesis of [14C]pyridoxal 5′-phosphate from [14C]pyridoxine

✍ Scribed by Toshiharu Yagi; Masashi Takasugi; Shinpei Yamamoto; Mitsuhiro Nozaki

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
461 KB
Volume
158
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new enzymatic method for the synthesis of ['4C]pyridoxal 5'-phosphate is presented.

[ r4C]Pyridoxal 5'-phosphate was synthesized from [ "C]pyridoxine through the successive actions of pyridoxal kinase and pyridoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, ['4C]pyridoxine. and both enzymes. ['4C]Pyridoxal 5'-phosphate was isolated by w-aminohexyl-Sepharose 6B column chromatography. The overall yield of the product was more than 60%, starting from 550 nmol of ['4C]pyridoxine. The radiochemical purity of the products, as determined by thin-layer and ion-exchange chromatography, was greater than 98%. o 1986 Academic Press. Inc.

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