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A Dependable Method for the Synthesis of [14C]Trehalose

✍ Scribed by B.U. Stambuk; J.H. Crowe; L.M. Crowe; A.D. Panek; P.S. Dearaujo

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
353 KB
Volume
212
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new method for the preparation of (\left[{ }^{14} \mathrm{C}\right]) trehalose was developed, based on the ability of yeast cells to accumulate trehalose under stress. The method is simple and reliable. It utilizes a yeast strain in which the gene that encodes for phosphoglucoisomerase has been deleted. Thus, exogenously supplied glucose is not metabolized, but is instead converted to trehalose. The (\left[{ }^{14} \mathrm{C}\right.) ]trehalose obtained is pure, it is hydrolyzed by trehalase, and it is not susceptible to the action of (\alpha)-glucosidase. The yield of this method is in the order of (35 %) of the (\left[{ }^{14} \mathrm{C}\right]) glucose supplied. (1993 Academic Press, Inc.

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