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An enzymatic method for the preparation of L- [1-14C] glycerol

✍ Scribed by P. F. Blackmore; M. G. Clark; J. F. Williams

Publisher
John Wiley and Sons
Year
1972
Tongue
French
Weight
211 KB
Volume
8
Category
Article
ISSN
0022-2135

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✦ Synopsis

B i o l o g i c a l l y l a b e l l e d [l-14C) g l y c e r o l ( L -[ l -' " C ) g l y c e r o l ) 048 prepared by a two s t e p p r o c e s s . S t e p one i n v o 1 v e d t h e p r e p a r a t i o n of [I -C

] g 2 y ce r o 1 3 -p ho sp ha t e from l4C02 and D-ribuloee 1,5

-diphoephate u s i n g t h e enzymes, r i b u l o a e d i p h o e p h a t e c a r b o z y l a s e , phoephoglycer a t e k i n a e e , g l y c e r a l d e h y d e 3-phosphate dehydrogenaee, t r i o s e phosphate ieomerase and L-a-gtycerophosphate dehydrogenase. S t e p two i n v o l v e d t h e c o n v e r s i o n o f (Z-14c] g l y c e r o l 3-phosphate t o (1-14C) g l y c e r o l , by e n z y m a t i c dephosphory l a t i o n . C i f i C a c t i v i t y of g t y c e r o t and i t s d e r i v a t i v e s , namely g l y c e r o l t r i b e n z o a t e and g l y c e r o l p -n i t r o b e n s o a t e , t h e compound was concluded t o be p u r e . Degradation by me-thod8 which d i s c r i m i n a t e between DL-( l -~4 C ) g l y c e r o 2 , D-[ Z -' " C ] g l y c e r o l and L -( 2 -1 4 C ] g l y c e r o Z i n d i c a t e d t h e compound t o be L -( l -' 4 C ] g l y c e r o l .

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