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The enzymatic preparation of L(+)-3-hydroxy(3-14C)butyrate

✍ Scribed by George Dombrowski Jr.; Kuen-Lan Chao; Kenneth R. Swiatek

Publisher
John Wiley and Sons
Year
1980
Tongue
French
Weight
162 KB
Volume
17
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

L(+)‐3‐hydroxy(3‐^14^C)butyrate was prepared by treating the D, L‐racemic mixture with the stereospecific D‐(−)‐3‐hydroxybutyrate dehydrogenase enzyme. The unfavorable equilibrium position of the dehydrogenase was overcome by the addition of acetaldehyde and alcohol dehydrogenase. The D‐isomer was converted by this treatment to acetoacetate. The unreacted L(+)‐3‐hydroxy(3‐^14^C)butyrate was then isolated by preparative silica gel chromatography in an overall yield of 65%.

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