An improved microfluorometric enzymatic assay for the determination of ammonia
โ Scribed by Barry L. Nazar; Anton C. Schoolwerth
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 395 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
The methods described in this paper are based on h e uricase catalyzed oxidation of uric acid to allantoine and hydrogen p x i d e . By making use of the catalytic activity of peroxidase the generated H f i is measured either spectrophotomedcally with 3 -m e t h y l -b e n z o W b 2 + m hydrazone (M
A method is described for the determination of the enzyme-catalyzed synthesis of y-glutamylmethylamide (GMAD). The assay involves conversion of '"C-labeled methylamine to ["'C] CMAD, addition of cold alcohol to stop the reaction, and plating an aliquot of the reaction mixture on planchets with alkal