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An assay for the enzymatic synthesis of γ-glutamylmethylamide

✍ Scribed by M.E. Levitch

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
238 KB
Volume
82
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for the determination of the enzyme-catalyzed synthesis of y-glutamylmethylamide (GMAD). The assay involves conversion of '"C-labeled methylamine to ["'C] CMAD, addition of cold alcohol to stop the reaction, and plating an aliquot of the reaction mixture on planchets with alkali to volatilize the unreacted methylamine. The nonvolatile GMAD on the planchet is then counted. 463

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