An enzymatic assay for activity of lipoprotein lipase
β Scribed by Laura A. Woollett; Donald C. Beitz; Ross L. Hood; Simon Aprahamian
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 395 KB
- Volume
- 143
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Monitoring of the elution from a high-performance gel chromatography column by a spectrophotometer, a low-angle laser light-scattering photometer and a precision differential refractometer as a versatile way to determine protein molecular weight.
## Abstract Lipoprotein lipase (LPL) is a key enzyme which regulates the plasma triglyceride concentration by hydrolyzing triglycerides in chylomicrons and veryβlowβdensity lipoprotein (VLDL). The activity of LPL was conventionally analyzed using radioβlabeled residues or direct sandwichβELISA. An