๐”– Bobbio Scriptorium
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Adaptation of an enzymatic fluorescence assay for l-glutamic acid decarboxylase

โœ Scribed by Rainer Wolf; Harald Klemisch

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
405 KB
Volume
192
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

The activity of L-glutamic acid decarboxylase (GAD) is commonly estimated by several radiometric methods, whereas a fluorimetric assay based on an enzymatic formation of NADPH as described by Y. Okada and C. Shimada [(1975) Brain Res. 98, 202-206] has been given little attention in biochemical and pharmacological investigations. A simple modification of this assay is presented to permit rapid and sensitive GAD measurements in unpurified tissue homogenates. This method, employing a linear NADPH standard curve, is demonstrated to be a valid assay system for a pharmacological approach using 3-mercaptopropionic acid.

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