An improved assay for the determination of phospholipase C activity
β Scribed by Markus A. Durban; Uwe T. Bornscheuer
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 200 KB
- Volume
- 109
- Category
- Article
- ISSN
- 1438-7697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We have developed an improved assay method to measure sphingomyelinase activity and to detect this enzyme separated on polyacrylamide gels. The assay of sphingomyelinase activity involved immobilizing \(\left[\mathbf{N}\right.\)-methyl \(\left.{ }^{14} \mathrm{C}\right]\) sphingomyelin on polyvinyld
We developed a specific spectrophotometric assay for the quantitative determination of phospholipase D-catalyzed transphosphatidylation activity. The assay measures p-nitrophenol liberated by phospholipase D-catalyzed reaction of phosphatidyl-p-nitrophenol and ethanol in an aqueous-organic emulsion
## Abstract An improved method for separation of tritiated benzo[__a__]pyrene from tritiated water in the arylβ4βhydroxylase assay is presented. Quantitative retention of benzo[__a__]pyrene was obtained with mixed cellulose acetate and nitrate filters. Enzymatic activities obtained with this modifi