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An improved method for the determination of aryl-4-hydroxylase activity

✍ Scribed by William J. Lindblad; Eugene Jackim

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
206 KB
Volume
1
Category
Article
ISSN
0260-437X

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✦ Synopsis

Abstract

An improved method for separation of tritiated benzo[a]pyrene from tritiated water in the aryl‐4‐hydroxylase assay is presented. Quantitative retention of benzo[a]pyrene was obtained with mixed cellulose acetate and nitrate filters. Enzymatic activities obtained with this modification agreed well with those determined using the standard charcoal column extraction. This modification greatly reduces the time required to perform this assay, and allows for the determination of enzyme activity in a large number of samples.

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