A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100Β°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)\*. Determination of hydroxyproline by the modified method does
An improved method for determination of hydroxyproline in protein hydrolyzates
β Scribed by Franca Serafini-Cessi; C. Cessi
- Publisher
- Elsevier Science
- Year
- 1964
- Tongue
- English
- Weight
- 126 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
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A continuous automatic method involving alkaline hydrolysis, neutralization with sulfuric acid, and specific detection of 4-hydroxyproline is described. The main parameters of the method are studied. This technique is capable of detecting 10 pmoliliter of Chydroxyproline linked in peptidic sequence
## Methods Reagents. Bz-Arg-NPhNO, was obtained from Seikagaku Kogyo Co., Ltd. Phenylmethylsulfonyl fluoride was purchased from Sigma. All other chemicals were reagent grade and commercially available. p-Dimethylaminobenzaldehyde reagent was prepared as follows: Concentrated sulfuric acid, 2.74 ml