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An improved method for determination of hydroxyproline in protein hydrolyzates

✍ Scribed by Franca Serafini-Cessi; C. Cessi


Publisher
Elsevier Science
Year
1964
Tongue
English
Weight
126 KB
Volume
8
Category
Article
ISSN
0003-2697

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A convenient modification of the method
✍ W. GalasiΕ„ski; Anna Gadek; Anna Ratkiewicz; W. Rzeczycki πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 336 KB

A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100Β°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)\*. Determination of hydroxyproline by the modified method does

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✍ G. Szymanowicz; G. Laurain πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 270 KB

A continuous automatic method involving alkaline hydrolysis, neutralization with sulfuric acid, and specific detection of 4-hydroxyproline is described. The main parameters of the method are studied. This technique is capable of detecting 10 pmoliliter of Chydroxyproline linked in peptidic sequence

An improved method for determining the a
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## Methods Reagents. Bz-Arg-NPhNO, was obtained from Seikagaku Kogyo Co., Ltd. Phenylmethylsulfonyl fluoride was purchased from Sigma. All other chemicals were reagent grade and commercially available. p-Dimethylaminobenzaldehyde reagent was prepared as follows: Concentrated sulfuric acid, 2.74 ml