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An automatic method of 4-hydroxyproline determination convenient for large series

✍ Scribed by G. Szymanowicz; G. Laurain

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
270 KB
Volume
113
Category
Article
ISSN
0003-2697

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✦ Synopsis

A continuous automatic method involving alkaline hydrolysis, neutralization with sulfuric acid, and specific detection of 4-hydroxyproline is described. The main parameters of the method are studied. This technique is capable of detecting 10 pmoliliter of Chydroxyproline linked in peptidic sequence with acceptable repeatability (SD/mean = 0.05). The automatic alkaline hydrolysis yield is 67% for soluble type I collagen. The method enables the analysis of 500 samples in 24 h.

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