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A convenient modification of the method for hydroxyproline determination in proteins

✍ Scribed by W. Galasiński; Anna Gadek; Anna Ratkiewicz; W. Rzeczycki

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
336 KB
Volume
85
Category
Article
ISSN
0003-2697

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✦ Synopsis

A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)*. Determination of hydroxyproline by the modified method does not require any additional chromatographic purification, standardizes conditions of the assay, and increases the yield in a number of routine assays.

📜 SIMILAR VOLUMES

An automatic method of 4-hydroxyproline
An automatic method of 4-hydroxyproline determination convenient for large series
✍ G. Szymanowicz; G. Laurain 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 270 KB

A continuous automatic method involving alkaline hydrolysis, neutralization with sulfuric acid, and specific detection of 4-hydroxyproline is described. The main parameters of the method are studied. This technique is capable of detecting 10 pmoliliter of Chydroxyproline linked in peptidic sequence