Modifications of a specific assay for hydroxyproline in urine
β Scribed by Kari I. Kivirikko; Ossi Laitinen; Darwin J. Prockop
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 434 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100Β°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)\*. Determination of hydroxyproline by the modified method does
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We developed a dye-binding method for albumin in urine based on bis (3β²,3β³-diiodo-4β²4β³-dihydroxy-5β²5β³-dinitrophenyl)-3,4,5,6tetrabromosulfonphthalein (DIDNTB), a dye that has a higher chemical sensitivity and specificity for albumin when compared to two other commonly used dyes. We prepared urine di