✦ LIBER ✦

An improved method for determining the activity of benzoyl-arginine p-nitroanilide-hydrolyzing enzyme in crude tissue extracts

✍ Scribed by Hideo Nakagawa; Katsuro Shuto; Susumu Tsurufuji

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 295 KB
Volume: 87
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90592-4

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✦ Synopsis

Methods

Reagents. Bz-Arg-NPhNO, was obtained from Seikagaku Kogyo Co., Ltd. Phenylmethylsulfonyl fluoride was purchased from Sigma. All other chemicals were reagent grade and commercially available. p-Dimethylaminobenzaldehyde reagent was prepared as follows: Concentrated sulfuric acid, 2.74 ml, was slowly added to 100 ml of absolute alcohol in a

📜 SIMILAR VOLUMES

Spectral data for modified assay of benz

Spectral data for modified assay of benzoyl-arginine p-nitroanilide-hydrolysing enzymes in crude extracts

✍ Colin W. Ward 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 229 KB

## Data for Modified Assay of Benzoyl-Arginine p-Nitroanilide-Hydrolysing Enzymes in Crude Extracts The resolution of complex mixtures of proteolytic enzymes is greatly simplified by the use of specific, preferably chromogenic, substrates. The assay is simplified further if the chromogenic substr