An assay system for the detection of phospholipase C activity
โ Scribed by Markus Durban; Uwe Bornscheuer
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 94 KB
- Volume
- 105
- Category
- Article
- ISSN
- 1438-7697
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๐ SIMILAR VOLUMES
We developed a specific spectrophotometric assay for the quantitative determination of phospholipase D-catalyzed transphosphatidylation activity. The assay measures p-nitrophenol liberated by phospholipase D-catalyzed reaction of phosphatidyl-p-nitrophenol and ethanol in an aqueous-organic emulsion
We have developed an improved assay method to measure sphingomyelinase activity and to detect this enzyme separated on polyacrylamide gels. The assay of sphingomyelinase activity involved immobilizing \(\left[\mathbf{N}\right.\)-methyl \(\left.{ }^{14} \mathrm{C}\right]\) sphingomyelin on polyvinyld