We developed a specific spectrophotometric assay for the quantitative determination of phospholipase D-catalyzed transphosphatidylation activity. The assay measures p-nitrophenol liberated by phospholipase D-catalyzed reaction of phosphatidyl-p-nitrophenol and ethanol in an aqueous-organic emulsion
โฆ LIBER โฆ
A spectrophotometric method for the assay of phospholipase D activity
โ Scribed by George M. Carman; Anthony S. Fischl; Maureen Dougherty; Gerhard Maerker
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 258 KB
- Volume
- 110
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A Spectrophotometric Assay for the Trans
โ
Tairo Hagishita; Masanobu Nishikawa; Tadashi Hatanaka
๐
Article
๐
1999
๐
Elsevier Science
๐
English
โ 77 KB
Carbonothioate Phospholipids as Substrat
โ
Lin Yu; Robert J. Ternansky; Jackie F. Crisologo; Julia Chang; Brian L. Baker; S
๐
Article
๐
1998
๐
Elsevier Science
๐
English
โ 119 KB
Continuous spectrophotometric assay of p
โ
Yutaka Hirashima; Marianne S. Jurkowitz-Alexander; Akhlaq A. Farooqui; Lloyd A.
๐
Article
๐
1989
๐
Elsevier Science
๐
English
โ 499 KB
A Spectrophotometric Method of Assay for
โ
Worrell, Lee ;Booth, Roger E.
๐
Article
๐
1953
๐
Elsevier
โ 378 KB
๐ 1 views
Improved spectrophotometric methods for
โ
Andrew Williams; Steven V. Hill; Ibrahim T. Ibrahim
๐
Article
๐
1981
๐
Elsevier Science
๐
English
โ 256 KB
Conductimetric Assays for the Hydrolase
โ
M. Mezna; A.J. Lawrence
๐
Article
๐
1994
๐
Elsevier Science
๐
English
โ 484 KB
Measurement of solution electrical conductance (conductimetry) is a simple direct assay method for the protogenic, hydrolytic reactions catalyzed by all phospholipase enzymes. The technique is especially suitable for assay of phospholipase D (PLD) enzymes where cleavage of zwitterionic substrates re