We developed a specific spectrophotometric assay for the quantitative determination of phospholipase D-catalyzed transphosphatidylation activity. The assay measures p-nitrophenol liberated by phospholipase D-catalyzed reaction of phosphatidyl-p-nitrophenol and ethanol in an aqueous-organic emulsion
Continuous spectrophotometric assay of phospholipase A2 activity hydrolyzing plasmalogens using coupling enzymes
โ Scribed by Yutaka Hirashima; Marianne S. Jurkowitz-Alexander; Akhlaq A. Farooqui; Lloyd A. Horrocks
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 499 KB
- Volume
- 176
- Category
- Article
- ISSN
- 0003-2697
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