An enzymatic radioactive assay to determine ribose 1-phosphate in tissues
✍ Scribed by Pier Luigi Ipata; Marcella Camici
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 238 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Ribose l-phosphate has been measured in rat tissues by an enzymatic radioactive assay. The sugar phosphate is converted into ["Clinosine via the two following combined reactions: ribose l-phosphate + [Wladenine = ['*C]adenosine + phosphate (adenosine phosphorylase); ['*C]adenosine + H,O + [Wlinosine + NH, (adenosine deaminase). Tissue extracts are incubated in the presence of excess [%]adenine. The radioactivity of inosine, separated by a thin-layer chromatographic system, is a measure of ribose l-phosphate present in tissue extracts. Liver was found to contain the highest level of ribose l-phosphate (ca. 800 nmoYg wet wt).