It is shown that the contribution of the gel itself to amino acid compositions of proteins eluted from polyacrylamide gels is significant (especially at low protein-to-gel ratios), linearly related to the volume of the gel slices eluted, and, when applied as a correction to amino acid composition da
Amino acid composition of myelin proteins recovered from polyacrylamide gels
β Scribed by Marjorie B. Lees; Sally A. Paxman
- Book ID
- 111167329
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- English
- Weight
- 348 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0022-3042
No coin nor oath required. For personal study only.
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A procedure which allows quantitative determination of the amino acid composition of stained proteins separated by sodium dodecyl sulfate (SDS)-gel electrophoresis using ninhydrin is described. The Coomassie-protein-SDS complex extracted from the gel is dialyzed, lyophylized, and subjected to amino