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Amino acid composition of proteins eluted from polyacrylamide gels: Background considerations

✍ Scribed by William E. Brown; Gary C. Howard

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 334 KB
Volume: 101
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90189-x

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✦ Synopsis

It is shown that the contribution of the gel itself to amino acid compositions of proteins eluted from polyacrylamide gels is significant (especially at low protein-to-gel ratios), linearly related to the volume of the gel slices eluted, and, when applied as a correction to amino acid composition data, results in an enhancement of the composition determination.

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