A method for electrophoretic elution of proteins from polyacrylamide gel slices is described. Eluted proteins were retained by a discontinuous conductivity gradient (M. Otto and M. Snejdárková, Anal. Biochem. 111, 111-114 (1981)). The method has been adapted to slices from slab gels and gels that ha
Two methods for electrophoretic elution of proteins from polyacrylamide gels
✍ Scribed by Per Kårsnäs; Paul Roos
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 437 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
A method for recovery of polypeptidcs from polyacrylamide gels by electrophoretic elution in a commercially available concentrator, the Amicon-Centricon sample reservoir, has been devised. The recoveries were greater than 90% with four different polypeptides tested ( 12.5 to 80 kDa). After elution,
It is shown that the contribution of the gel itself to amino acid compositions of proteins eluted from polyacrylamide gels is significant (especially at low protein-to-gel ratios), linearly related to the volume of the gel slices eluted, and, when applied as a correction to amino acid composition da