✦ LIBER ✦

Recovery of polypeptides from polyacrylamide gels by electrophoretic elution in a centrifugation concentrator

✍ Scribed by Setsunosuke Ihara; Haruo Suzuki; Masaya Kawakami

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 323 KB
Volume: 166
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90584-7

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✦ Synopsis

A method for recovery of polypeptidcs from polyacrylamide gels by electrophoretic elution in a commercially available concentrator, the Amicon-Centricon sample reservoir, has been devised. The recoveries were greater than 90% with four different polypeptides tested ( 12.5 to 80 kDa). After elution, sample concentration or salt exchange can be carried out without sample transfer. There were no loss ofsample during the postelution procedures when the elution buffer was replaced by 0.01 or 0.058, sodium dodecyl sulfate.

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