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Electrophoretic elution of proteins from polyacrylamide gel slices

✍ Scribed by Peter Strålfors; Per Belfrage

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
452 KB
Volume
128
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for electrophoretic elution of proteins from polyacrylamide gel slices is described. Eluted proteins were retained by a discontinuous conductivity gradient (M. Otto and M. Snejdárková, Anal. Biochem. 111, 111-114 (1981)). The method has been adapted to slices from slab gels and gels that have been stained and destained. Proteins were eluted as their sodium dodecyl sulfate complexes. Minute amounts of proteins (0.1 microgram) were recovered in high yield (85-95%) in 2 h in less than 0.1 ml volume.

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