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A modified method for amino acid analysis with ninhydrin of coomassie-stained proteins from polyacrylamide gels

✍ Scribed by K. Sreekrishna; Chris E. Jones; Karl A. Guetzow; M. Renuka Prasad; Vasudev C. Joshi

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 213 KB
Volume: 103
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90235-3

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✦ Synopsis

A procedure which allows quantitative determination of the amino acid composition of stained proteins separated by sodium dodecyl sulfate (SDS)-gel electrophoresis using ninhydrin is described. The Coomassie-protein-SDS complex extracted from the gel is dialyzed, lyophylized, and subjected to amino acid analysis after hydrolysis with constant boiling HCI. By this method it is possible to quantiate the amino acids Asx,

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