We describe here a protein staining method in polyacrylamide gels with a new staining dye, 1-(2-hydroxy4-sulfo-1-naphthylazo)-2-hydroxy-3-naphthoic acid (calconcarboxylic acid, NN). This method can be performed by both simultaneous and postelectrophoretic staining techniques. Simultaneous staining u
Desalting of proteins, peptides, and amino acids on polyacrylamide gel
β Scribed by Alice N. Schwartz; Burton A. Zabin
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 342 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
It is shown that the contribution of the gel itself to amino acid compositions of proteins eluted from polyacrylamide gels is significant (especially at low protein-to-gel ratios), linearly related to the volume of the gel slices eluted, and, when applied as a correction to amino acid composition da
This is the first of five books in the *Amino Acids, Peptides and Proteins in Organic Synthesis* series. Closing a gap in the literature, this is the only series to cover this important topic in organic and biochemistry. Drawing upon the combined expertise of the international "who's who" in amino
A novel method has been developed for the rapid desalting of proteins and peptides for electrospray ionization mass spectrometry (ESI-MS). Protein and peptide solutions having high salt concentrations were infused into a microdialysis tube which was directly interfaced with a micro-ESI source. The m
This is the first of five books in the *Amino Acids, Peptides and Proteins in Organic Synthesis* series. Closing a gap in the literature, this is the only series to cover this important topic in organic and biochemistry. Drawing upon the combined expertise of the international "who's who" in amino