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On-line Microdialysis Desalting for Electrospray Ionization-Mass Spectrometry of Proteins and Peptides

✍ Scribed by Qinyuan Wu; Chuanliang Liu; Richard D. Smith

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
449 KB
Volume
10
Category
Article
ISSN
0951-4198

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✦ Synopsis

A novel method has been developed for the rapid desalting of proteins and peptides for electrospray ionization mass spectrometry (ESI-MS). Protein and peptide solutions having high salt concentrations were infused into a microdialysis tube which was directly interfaced with a micro-ESI source. The microdialysis tube was surrounded by a counter-current flow of a buffer solution consisting of 10 mM ammonium acetate. Desalting was completed in one to four minutes for samples containing up to 2 5 0 m NaCl, which would otherwise have required several hours using alternative off-line desalting schemes. The on-line desalting method also increased the signal-to-noise ratio by a factor of more than 40 compared with that obtained without desalting. Detection of subpicomole samples of apomyoglobin having a high salt concentration was also achieved. Initial results for apomyoglobin, albumin and angiotensin I indicate broad applicability of the method.

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