𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Sensitive Detection of Native Proteins Using Extractive Electrospray Ionization Mass Spectrometry

✍ Scribed by Huanwen Chen; Shuiping Yang; Ming Li; Bin Hu; Jianqiang Li; Jiang Wang


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
431 KB
Volume
122
Category
Article
ISSN
0044-8249

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Charge concentration in electrospray ion
✍ R. S. Ramsey; S. A. McLuckey πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 121 KB πŸ‘ 1 views

A novel method of detecting proteins at low concentration based upon electrospray ionization᎐mass spectrometry and gas-phase proton transfer reactions is described. In this technique the signal that is spread over a broad mass range due to the distribution of multiply charged ions generated in elect

Detection of microcystins using electros
✍ Barbara Ells; Kenneth Froese; Steve E. Hrudey; Randy W. Purves; Roger Guevremont πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 101 KB

A combination of electrospray ionization, high-field asymmetric waveform ion mobility spectrometry, and mass spectrometry (ESI-FAIMS/MS) was used to analyze standard solutions of microcystins-LR, -RR, and -YR. The ability of FAIMS to separate ions in the gas phase reduced the amount of background in

Characterization of a Mutant Recombinant
✍ Mark J. Raftery; Craig A. Harrison; Carolyn L. Geczy πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 118 KB

Two recombinant proteins derived by thrombin cleavage of a fusion protein between glutathione-S-transferase and CP10 (Chemotactic protein 10 kDa) were seperated by C4 reversed-phase high-performance liquid chromatography (RP-HPLC). Both proteins were recognised by a polyclonal antibody to native CP1

Analysis of Hydrophobic Proteins and Pep
✍ Johann Schaller; Bernhard Christian Pellascio; Urs Peter Schlunegger πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 465 KB πŸ‘ 2 views

Bacterioopsin (BO) from Halobacterium halobium, as well as its V8-protease and CNBr fragments from the C-terminal region, were used to establish appropriate conditions for the mass determination of membrane proteins and peptides by electrospray mass spectrometry (ESI-MS). Of the tested solvents neat

Unravelling seemingly complex chemistry
✍ Ray Bakhtiar; Cornelis E. C. A. Hop πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 195 KB πŸ‘ 1 views

Since its discovery, electrospray ionization mass spectrometry (ESI-MS) has provided valuable information on structurally specific biomolecular interactions, including DNA-drug, enzyme-inhibitor, DNAprotein and protein-protein interactions. In addition, this technique has emerged as a powerful tool