๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Charge concentration in electrospray ionization: Mass spectrometry for sensitive detection of proteins

โœ Scribed by R. S. Ramsey; S. A. McLuckey

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
121 KB
Volume
9
Category
Article
ISSN
1040-7685

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โœฆ Synopsis

A novel method of detecting proteins at low concentration based upon electrospray ionizationแŽmass spectrometry and gas-phase proton transfer reactions is described. In this technique the signal that is spread over a broad mass range due to the distribution of multiply charged ions generated in electrospray is focused into a singly-charged, low mass ion. The experiments performed on a quadrupole ion trap involve accumulating the ions of the biopolymer, allowing these ions to react with a strong neutral base, and recording the intensity of the singly-charged product in a final mass analysis scan. Concentration detection limits for high-mass proteins may be improved by over two orders of magnitude using this approach. Targeted compounds can be detected at low nanomolar levels using moderate reaction times with a strong base.

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